联系方式

  • 职  称: 副教授
  • 所在部门: 生物物理所
  •  办公室: 天演楼403
  • 联系电话: 0931-8912563
  • 传真号码:
  • 电子邮件: heyx@
  • 个人主页: http://qb3.berkeley.edu/qb3/tang-hyx.cfm
学习经历

2011/02-2013/02,加州大学伯克利分校,生物化学与分子生物学系(定量生物学研究所QB3),博士后

2005/09-2010/12,中国科大,生化与分子生物系,博士

2001/09-2005/07,中国科大,生命科学院,学士

 

工作经历

2013/03-今,兰州大学生命科学学院,副教授

社会工作

研究方向

随着抗生素的广泛使用,细菌的多重耐药性对人类健康造成了巨大的危害。我们实验室主要致力于研究铜绿假单胞菌和荧光假单胞的多重耐药性产生的机制。目前已建立较为成熟的蛋白质组学和结构生物学平台,欢迎对生物信息学、蛋白质组学及微生物学感兴趣的同学报考本实验室研究生!

项目成果

国家自然科学基金委面上项目:吲哚调节荧光假单胞菌耐药性及莫匹罗星合成的分子机制研究(31971422),执行时间:2020/01-2023/12

国家自然科学基金委面上项目:毒素-抗毒素MqsRA调控荧光假单胞菌抗药性的分子机制研究(31972319),执行时间:2020/01-2023/12

国家自然科学基金委面上项目:植物根际促生菌Pseudomonas-fluorescems 2P24感受与影响类黄酮的分子机制研究,执行时间:2018/01-2021/12

国家自然科学基金委青年项目:荧光假单胞杆菌中的抗生素DAPG合成酶的晶体结构及其催化机理,2014/01-2016/12

甘肃省自然科学基金面上项目:黄酮类物质对雄激素受体信号转导的拮抗机制研究,2019年/01-2020/12

国家自然科学基金委面上项目:2,4-DAPG及MAPG作为荧光假单胞菌信号分子的作用机制,执行时间:2020/01-2023/12 承担经费:22万

荣誉、获奖

2019年甘肃医学科技奖一等奖,排名5/9

2019年甘肃省科技进步二等奖,排名7/10

教学及指导研究生情况

讲授《生物信息学》

在读研究生 4人

访问学生   2人

毕业硕士6人,在兰州大学、上海交大医学院、清华大学、西湖大学、湖南大学及景杰生物等高校或公司读博或工作。

发表论文及专著

2013年后发表文章(#通讯作者):


	
Guo DD*, Luo LM* et al. He YX# The regulator PltZ regulates a novel antibiotic efflux pump PltIJKNOP of Pseudomonas aeruginosa ATCC 27853 in response to 
the antimicrobial 2,4-diacetylphloroglucinol. FRONT MICROBIOL. 2020 Revision submitted

Yu XQ*, Yan X*. et al. He YX# Flavonoids repress production of antifungal 2,4-DAPG but potentially facilitate root colonization of the rhizobacterium Pseudomonas fluorescens.
 Environ Microbiol. 2020 in press

Zhang SP, Wang Q, Quan SW, Yu XQ, Wang Y, Guo DD, Peng L, Feng HY#, He YX# Type II toxin-antitoxin system in bacteria: activation, function and mode of action.
Biophysical Reports. 2020 in press

Na M, Zhang S, Liu J, Ma S, Han Y, Wang Y, He YX, Chen H, Chen X. Determination of pathogenic bacteria-Bacillus anthrax spores in environmental samples by
ratiometric fluorescence and test paper based on dual-emission fluorescent silicon nanoparticles. J Hazard Mater. 2020 Mar 15;386:121956

Zhang ZY, Bai HH, Guo Z, Li HL, He YT, Duan XL, Suo ZW, Yang X, He YX, Hu XD. mGluR5/ERK signaling regulated the phosphorylation and function of glycine
receptor α1ins subunit in spinal dorsal horn of mice. PLoS Biol. 2019 Aug 21;17(8)

Han JT*, Zhang SP*, Jia WJ, Zhang Z, Wang Y, He YX#. Discovery and structural analysis of a phloretin hydrolase from the opportunistic human pathogen Mycobacterium abscessus. FEBS Journal. 2019 

Wang Y*, Zhang SP*, Zhang MY, Kempher ML, Guo DD, Han JT, Tao X, Wu Y, Zhang LQ, He YX#. The antitoxin MqsA homolog in Pseudomonas fluorescens 2P24 has a rewired regulatory circuit through evolution. Environ Microbiol. 2019 Jan 24.

Zhao JH, Chen JH, Wang Y, Wang ZP, He YX#. The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical
characterization and crystallographic data. Acta Crystallogr F Struct Biol Commun. 2018 Apr 1;74(Pt 4):231-235.

Yan X, Yang R, Zhao RX, Han JT, Jia WJ, Li DY, Wang Y, Zhang N, Wu Y, Zhang LQ#, He YX#. The transcriptional regulator PhlH modulates 2,4-diacetylphloroglucinol biosynthesis in response to the biosynthetic intermediate and end product. Appl Environ Microbiol. 2017 Aug 18

Li L, Zhu Y, Zhou S, An X, Zhang Y, Bai Q, He YX#, Liu H#, Yao XJ#. Experimental and Theoretical Insights into the Inhibition Mechanism of Prion Fibrillation by Resveratrol and its Derivatives. ACS Chem Neurosci. 2017 Aug 17

Sun C, Yan K, Han JT, Tao L, Lv MH, Shi T, He YX, Wierzba M, Tax FE, Li J.Scanning for New BRI1 Mutations via TILLING Analysis. Plant Physiol. 2017 Jul;174(3):1881-1896.

Nan Q, Qian D, Niu Y, He YX, Tong S, Niu Z, Ma J, Yang Y, An L, Wan D, Xiang Y. Plant Actin-depolymerizing Factors Possess Opposing Biochemical Properties Arising From Key Amino Acid Changes Throughout Evolution. Plant Cell. 2017 Jan 25

Bo Xin, Geng Wu, Kunzhi Zhang, YX He, Hongzhi Tang, Chao Gao, Ping Xu and Cuiqing Ma Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa Bioresour. Bioprocess. (2016) 3: 32.

Wu D, Tao X, Chen ZP, Han JT, Jia WJ, Zhu N, Li X, Wang ZP#, He YX#The environmental endocrine disruptor p-nitrophenol interacts with FKBP51, a positive regulator of androgen receptor and inhibits androgen receptor signaling in human cells. J Hazard Mater. 2016 Apr 15;307:193-201 (五年IF=5.277, JCR一区)

Zhang Z, Jiang YL, Wu Y, He YX#. Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):239-42.(IF=0.568, JCR四区)

Mu Y, Lian FM, Teng YB, Ao J, Jiang YL, He YX, Chen Y, Zhou CZ, Chen X. The N-terminal β-sheet of peroxiredoxin 4 in the large yellow croaker Pseudosciaena crocea is involved in its biological functions. PLoS One. 2013;8(2):e57061.(五年IF=4.015, JCR三区)

2013之前发表文章第一或共第一作者文章(*共同第一作者):

He YX, Zhang NN, Li WF, Jia N, Chen BY, Zhou K, Zhang J, Chen Y, Zhou CZ. N-Terminal domain of Bombyx mori fibroin mediates the assembly of silk in response to pH decrease. J Mol Biol. 2012 May 4;418(3-4):197-207.(五年IF=3.795, JCR二区)

Du Y*, He YX*, Zhang ZY, Yang YH, Shi WW, Frolet C, Di Guilmi AM, Vernet T, Zhou CZ, Chen Y. Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae. J Struct Biol. 2011 Apr;174(1):252-7.(五年IF=3.407, JCR二区)

He YX, Huang L, Xue Y, Fei X, Teng YB, Rubin-Pitel SB, Zhao H, Zhou CZ. Crystal structure and computational analyses provide insights into the catalytic mechanism of 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens. J Biol Chem. 2010 Feb 12;285(7):4603-11.(五年IF= 4.863,JCR二区)

He YX, Gui L, Liu YZ, Du Y, Zhou Y, Li P, Zhou CZ. Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly. Proteins. 2009 Jul;76(1):249-54.(五年IF=2.964,JCR三区)

Zhang WJ*, He YX*, Yang Z, Yu J, Chen Y, Zhou CZ. Crystal structure of glutathione-dependent phospholipid peroxidase Hyr1 from the yeast Saccharomyces cerevisiae. Proteins. 2008 Dec;73(4):1058-62.(五年IF=2.964,JCR三区)

其他文章:

Zhao MX, Jiang YL, He YX, Chen YF, Teng YB, Chen Y, Zhang CC, Zhou CZ. Structural basis for the allosteric control of the global transcription factor NtcA by the nitrogen starvation signal 2-oxoglutarate. Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12487-92.

Ma G, Yu J, Xiao Y, Chan D, Gao B, Hu J, He YX, Guo S, Zhou J, Zhang L, Gao L, Zhang W, Kang Y, Cheah KS, Feng G, Guo X, Wang Y, Zhou CZ, He L. Indian hedgehog mutations causing brachydactyly type A1 impair Hedgehog signal transduction at multiple levels. Cell Res. 2011 Sep;21(9):1343-57.

Kang Y, He YX, Zhao MX, Li WF. Structures of native and Fe-substituted SOD2 from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1173-8.

Yang JP, Ma XX, He YX, Li WF, Kang Y, Bao R, Chen Y, Zhou CZ. Crystal structure of the 30 K protein from the silkworm Bombyx mori reveals a new member of the β-trefoil superfamily. J Struct Biol. 2011 Jul;175(1):97-103.

Du Y, Shi WW, He YX, Yang YH, Zhou CZ, Chen Y. Structures of the substrate-binding protein provide insights into the multiple compatible solute binding specificities of the Bacillus subtilis ABC transporter OpuC. Biochem J. 2011 Jun 1;436(2):283-9.

Zhang X, Teng YB, Liu JP, He YX, Zhou K, Chen Y, Zhou CZ. Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1. Biochem J. 2010 Dec 15;432(3):445-50.

Du Y, He YX, Gaowa S, Zhang X, Chen Y, Zhang SC, Zhou CZ. Crystal structures of the apo and GDP-bound forms of a cupin-like protein BbDUF985 from Branchiostoma belcheri tsingtauense. Proteins. 2010 Sep;78(12):2714-9.

Luo M, Jiang YL, Ma XX, Tang YJ, He YX, Yu J, Zhang RG, Chen Y, Zhou CZ. Structural and biochemical characterization of yeast monothiol glutaredoxin Grx6. J Mol Biol. 2010 May 14;398(4):614-22.

Zhang NN, He YX, Li WF, Teng YB, Yu J, Chen Y, Zhou CZ. Crystal structures of holo and Cu-deficient Cu/Zn-SOD from the silkworm Bombyx mori and the implications in amyotrophic lateral sclerosis. Proteins. 2010 Jun;78(8):1999-2004.

Teng YB, Jiang YL, He YX, He WW, Lian FM, Chen Y, Zhou CZ. Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103. BMC Struct Biol. 2009 Oct 24;9:67.

Ma XX, Jiang YL, He YX, Bao R, Chen Y, Zhou CZ. Structures of yeast glutathione-S-transferase Gtt2 reveal a new catalytic type of GST family. EMBO Rep. 2009 Dec;10(12):1320-6.

Teng YB, Ma XX, He YX, Jiang YL, Du J, Xiang C, Chen Y, Zhou CZ. Crystal structure of Arabidopsis translation initiation factor eIF-5A2. Proteins. 2009 Nov 15;77(3):736-40.

Wang Y, He YX, Yu J, Zhou CZ. Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):651-3.

Huang L, Sun F, Liang C, He YX, Bao R, Liu L, Zhou CZ. Crystal structure of LZ-8 from the medicinal fungus Ganoderma lucidium. Proteins. 2009 May 1;75(2):524-7. doi: 10.1002/prot.22346.

Wang S, He YX, Bao R, Teng YB, Ye BP, Zhou CZ. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):847-50. 

参与翻译编写《英汉生物技术词典》,化学工业出版社

 

 

其它信息

欢迎对生物信息学、蛋白质组学及微生物学感兴趣的同学报考本实验室研究生!

更新:2020-10-18 | 访问:... | 二维码 | 维护教师主页