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何凯

个人简历

科研成果

教学情况

研究方向

论文专著

个人简历

基本信息

职称:教授

办公室地址:逸夫生物楼614

联系电话: 0931-8915209

邮箱:hekai@lzu.edu.cn

学习经历

2008  美国俄克拉荷马大学植物与微生物系  博士                             

2003  四川大学生命科学院  硕士 

2000  四川大学生命科学院  学士  

工作经历

2013至今     兰州大学生命科学学院  教授

2009-2013  美国加州大学伯克利分校植物与微生物系  博士后

2005-2008  美国俄克拉荷马大学  教学助理

2003-2008  美国俄克拉荷马大学  研究助理

社会工作

科研成果

主持科研项目:

  1. 国家自然科学基金面上项目“硝酸根离子通道SLAH3参与调控植物氮-钾平衡的分子机理”(No.32170280)2022-2025,58万;
  2. 国家自然科学基金面上项目“离子通道蛋白 SLAH3 调控植物对高铵和低 pH 胁迫响应的分子机理研究”、(No.31870235)2019-2022,60万;
  3. 国家自然科学基金面上项目“拟南芥受体激酶 BAK1 和 BKK1 介导细胞死亡分子机理”(No.31471305)2015-2018,80万;
  4. 中央高校基本科研业务费专项资金-自由探索-面上项目“BED1依赖 MAPK 途径调节植物花序发育”(lzujbky-2016-75)2016-2017,10万;
  5. 中央高校基本科研业务费专项资金-科研创新团队培育项目“植物免疫与逆境响应的分子机制”(lzujbky-2015-ct01)2015-2016,50万;
  6. 中央高校基本科研业务费专项资金-自由探索-重点项目“拟南芥蛋白激酶 SnRK1 的生物学功能研究”(lzujbky-2015-k17)2015-2016,14万;
  7. 中央高校基本科研业务费专项资金-自由探索-面上项目“拟南芥阴离子通道蛋白介导氮平衡的分子机理”(lzujbky-2014-84)2014-2015,10万。

参与科研项目:

  1. 国家作物转基因重大专项子课题,2016ZX08009-003-002,2016-2020,200万;
  2. 国家自然科学基金植物激素作用的分子机理重大研究集成项目“ BAK1 调控油菜素内酯(BR)信号转导及器官发育的分子机制”(91317311)2014-2015,200万。

教学情况

教学:

本科生《细胞信号转导》教学

研究生《Cell signal transduction》全英文教学

本科生校级、国家级科研训练

研究生招生:

硕士,遗传学(071007);博士,遗传学(071007

研究方向为植物分子遗传学、植物逆境适应分子机理、基因编辑

目前指导博士后1人,博士研究生6人,硕士研究生8人。

博士后合作:

欢迎近期毕业博士合作开展博士后研究工作,兰州大学提供博士后、师资博士后、萃英博士后以及青年研究员工作岗位

研究方向

本课题组主要关注植物逆境适应过程中的分子机制:

1)植物氮胁迫与氮信号(Nitrogen Stress and Signaling in Plants):氮素(Nitrogen, N)作为植物营养的三大要素之一,是构成植物体多种有机化合物的基本成分。植物主要以无机态氮:铵态氮(NH4+)、硝态氮(NO3-)的形式吸收氮素,而氮素会直接影响植物的各项生命活动。本团队致力于研究植物对氮素营养的感知、吸收、转运以及氮素信号应答机制,首次发现硝酸根离子外排通道SLAH3通过平衡植物硝态氮的吸收,在缓解植物铵盐毒害过程中发挥重要作用。主要研究方向包括三方面:A.植物对高铵氮源逆境适应的分子机理;B.植物对氮素的感知与信号转导机制;C.植物对氮素的塑性反应分子机理。

2)拟南芥类受体蛋白的生物学功能Functional Analyses of Receptor-Like Proteins, RLPs):细胞表面的受体激酶(Receptor-Like Kinases, RLKs)在细胞与周围环境之间的信号转导过程中起至关重要的作用。与类受体蛋白激酶不同,类受体蛋白只包含胞外识别域、跨膜域,通常缺乏胞内激酶结构域。本团队正在利用各种遗传学、分子生物学以及组学手段对RLP中尚未揭示功能的组分开展生物学功能研究。

论文专著

(*corresponding author; §contributed equally)

  • Xiao C., Sun, D., Liu B., Fang, X., Li P., Jiang Y., He M., Li J., Luan S., He, K.* (2022) Nitrate transporter NRT1.1 and anion channel SLAH3 form a functional unit to regulate nitrate-dependent alleviation of ammonium toxicity. Journal of Integrative Plant Biology, DOI: 10.1111/jipb.13239
  • Fang, X., Zhang, Y., Cheng, B., Luan, S.*, He, K.* (2021). Evidence for the involvement of AtPiezo in mechanical responses. Plant Signaling & Behavior,  DOI:10.1080/15592324.2021.1889252
  • Sun, D., Fang, X., Xiao, C., Ma, Z., Huang, X., Su, J., Li, J., Wang, J., Wang, S., Luan, S., He, K.* (2021) Kinase SnRK1.1 regulates nitrate channel SLAH3 engaged in nitrate-dependent alleviation of ammonium toxicity. Plant Physiology, DOI:10.1093/plphys/kiab057 

Commented in: Wang, P. (2021). Friend or foe: how nitrate antagonizes ammonium toxicity. Plant Physiology, kiab095. Advance online publication. https://doi.org/10.1093/plphys/kiab095

  • Fang, X., Liu, B., Shao, Q., Huang, X., Li, J., Luan, S.*, and He, K.* (2021) AtPiezo plays an important role in root cap mechanotrasdiction. International Journal of Molecular Sciences 22, 467.
  • Chen, L., Zhao, M., Wu, Z., Chen, S., Rojo, E., Luo, J., Li, P., Zhao, L., Chen, Y., Deng, J., Cheng, B., He, K., Gou, X., Li, J., Hou, S.* (2020) RNA polymerase II associated proteins regulate stomatal development through directly interacting with the stomatal transcription factors in Arabidopsis thaliana. New Phytologist, DOI:10.1111/nph.17004
  • Wu, Y., Gao, Y., Zhan, Y., Kui, H., Liu, H., Yan, L., Kemmerling, B., Zhou, J-M., He, K.*, Li, J.* (2020) Loss of the common immune coreceptor BAK1 leads to NLR-dependent cell death. Proc. Natl. Acad. Sci. USA 117(43), 27044-27053.
  • Lu, X., Shi, H., Ou, Y., Cui, Y., Chang, J., Peng, L., Gou, X., He, K., Li, J.* (2020) RGF1-RGI1, a peptide-receptor complex, regulates Arabidopsis root meristem development via a MPAK signaling cascade. Molecular Plant 13, 1594-1607.
  • Xun, Q., Wu, Y., Li, H., Chang, J., Ou, Y., He, K., Gou, X., Tax, F. E., Li, J.* (2020) Two receptor-like protein kinases, MUSTACHES and MUSTACHES-LIKE, regulate lateral root development in Arabidopsis thaliana, New Phytologist 227, 1157-1173.
  • Chang, J., Li, X., Fu, W., Wang, J., Yong, Y., Shi, H., Ding, Z., Kui, H., Gou, X., He, K., Li, J.* (2019) Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana. Cell Research 29(12), 984-993.
  • Li, H., Cai, Z., Wang, X., Li, M., Cui, Y., Cui, N., Yang, F., Zhu, M., Zhao, J., Du, W., He, K., Yi, J., Tax, F.E., Hou, S., Li, J., Gou, X.* (2019) SERK receptor-like kinases control division patterns of vascular precursors and ground tissue stem cells during embryo development in Arabidopsis. Molecular Plant 12(7), 984-1002.
  • Lv, M., Li, M., Chen, W., Wang, Y., Sun, C., Yin, H., He, K., and Li, J.* (2018) Thermal-enhanced bri1-301 instability reveals a plasma membrane protein quality control system in plants. Frontiers in Plant Science 9, 1620.
  • Cui, Y., Hu, C., Zhu, Y., Cheng, K., Li, X., Wei, Z., Xue, L., Lin, F., Shi, H., Yi, J., Hou, S., He, K., Li, J., Gou, X.* (2018) CIK Receptor kinases determine cell fate specification during early anther development in Arabidopsis. Plant Cell 30, 2383-2401.
  • Hu, C., Zhu, Y., Cui, Y., Cheng, K., Liang, W., Wei, Z., Zhu, M., Yin, H., Zeng, Li, Xiao, Y., Lv, M., Yi, J., Hou, S., He, K., Li, J., Gou, X.* (2018). A group of receptor kinases are essential for CLAVATA signaling to maintain stem cell homeostasis. Nature Plants 4, 205-211.
  • Wei, Z., Yuan,T., Tarkowská,D., Kim,J., Nam,H.G.,Novák,O., He, K., Gou, X. and Li, J.* (2017) Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4 and PIF5. Plant Physiology 174, 1260-1273.
  • Gao Y., Wu Y., Du J., Zhan Y., Sun D., Zhao J., Zhang S., Li J. and He K.* (2017) Both light-induced SA accumulation and ETI mediators contribute to the cell death regulated by BAK1 and BKK1. Frontiers in Plant Science 25;8:622.
  • Wei, Z., Yuan, T., Tarkowská, D., Kim, J., Nam, H.G., Novák, O., He, K., Gou, X. and Li, J. (2017) Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4 and PIF5. Plant Physiology 174(2):1260-1273
  • He, K.*, and Wu, Y. (2016) Receptor-Like Kinases and Regulation of Plant Innate Immunity. Enzymes, 40:105-142
  • Ou, Y., Lu, X., Zi, Q., Xun, Q., Zhang J., Wu, Y., Shi, H., Wei, Z., Zhao, B., Zhang, X., He, K., Gou, X., Li, C., Li, J. (2016) RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thalianaCell Research, 26(6), 686-698
  • Chen, L., Guan, L., Qian, P., Xu, F., Wu, Z., Wu, Y., He, K., Gou, X., Li, J., and Hou, S. (2016) NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016 Mar 17. pii: dev.129098.
  • Du, J., Gao, Y., Zan, Y., Zhang, S., Wu, Y., Xiao, Y., Zou, B., He, K., Gou, X., Li, G., Lin, H., Li, J. (2016) Nucleocytoplasmic trafficking is essential for BAK1 and BKK1-mediated cell-death control. Plant Journal 85(4), 520-531.
  • Wu, Y., Xun, Q., Guo, Y., Zhang, J., Cheng, K., Shi, T., He, K., Hou, S., Gou, X., Li, J. (2016) Genome-wide expression pattern analyses of the Arabidopsis leucine-rich repeat receptor-like kinases. Molecular Plant 9(2), 289-300.
  • Wu, W., Wu Y., Gao, Y., Li, M., Yin, H., Lv, M., Zhao, J., Li, J., He, K.* (2015) Somatic embryogenesis receptor-like kinase 5 in the ecotype Landsberg erecta of Arabidopsis is a functional RD LRR-RLK in regulating brassinosteroid signaling and cell death control. Frontiers in Plant Science 6:852. doi: 10.3389/fpls.2015.00852
  • Zheng X.§He K.§, Kleist T., Chen F., Luan S. (2015) Anion channel SLAH3 functions in nitrate-dependent alleviation of ammonium toxicity in Arabidopsis. Plant, Cell and Environment Mar;38(3):474-86
  • Hou C., Tian W., Kleist T., He K., Garcia V., Bai F., Hao Y., Luan S., Li L. (2014) DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes. Cell Research 2014 Feb 7. doi: 10.1038/cr.2014.14.
  • He K., Xu S., Li J. (2013) BAK1 directly regulates brassinosteroid perception and BRI1 activation. Journal of Integrative Plant Biology 55(12), 1264–1270. ( Cover article)
  • Lee SC., Lim CW., Lan W., He K., Luan S. (2013) ABA signaling in guard cells entails a dynamic protein-protein interaction relay from the PYL-RCAR family receptors to ion channels. Molecular Plant 6:528-38.
  • Gou, X.§, Yin, H.§He, K.§, Du, J., Yi., J., Xu, S. Lin, H., Clouse, SD., Li, J. (2012) Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling. PLoS Genetics 8(1): e1002452. doi:10.1371/journal.pgen.1002452.
  • Li J., Du J., He K., Gou X. (2011). Control of cell death by Receptor Kinases in Arabidopsis thaliana. In "Receptor-like Kinases in Plants: From Development to Defense (eds Birgit Kemmerling and Frans Tax)". Signaling and Communication in Plants (ed. Frantisek Baluska, Springer Publisher), Volume 13, pp79-91.
  • Yang, H., Gou, X., He, K., Xi, D., Du, J., Lin, H., Li, J. (2010) Arabidopsis BAK1 and BKK1 confer reduced susceptibility to Turnip Crinkle Virus. European Journal of Plant Pathology 127: 147-156.
  • Gou, X., He, K., Yang, H., Yuan, T., Lin, H., Clouse, S.D., Li, J. (2010) Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana. BMC Genomics 11(1):19
  • He, K., Gou, X., Powell, R.A., Yang, H., Yuan, T, Guo, Z., and Li, J.(2008) Receptor-like protein kinases, BAK1 and BKK1, regulate a light-dependent cell-death control pathway. Plant Signaling & Behavior 3 (10), 813-815.
  • ang, X., Kota, U., He, K., Blackburn, K., Li, J., Goshe, M.B., Huber, S.C., and Clouse, S.D.(2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase pair impacts early events in brassinosteroid signaling. Developmental Cell 15, 220-235.
  • He, K., Gou, X., Yuan, T., Lin, H., Asami, T., Yoshida, S., Russell, S.D., Li, J. (2007) BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell death pathways. Current Biology 17, 1109-1115.
  • Heese, A., Hann, D.R., Gimenez-ibanex, S., Jones, A., He, K., Li, J., Schroeder, J.I., Peck, S.C., Rathjen, J.P. (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proceedings of the National Academy of Sciences of the United States of America 104, 12217-12222.
  • Yuan, T., Fujioka, S., Takatsuto, S., Matsumoto, S., Gou, X., He, K., Russell, S.D., and Li, J.(2007) BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana. The Plant Journal 51 (2), 220–233.
最新更新时间: 2022-10-19