一.获批项目：

1.基于调控微生物代谢能耗的硝基苯降解调控开关的研究.甘肃省自然科学基金青年基金.2020/10-2022/11.

2.亚硝酸盐还原酶转录调控蛋白NsrR去阻遏机制研究.国家自然科学基金青年基金.2017/01-2019/12.

一. 教学情况：

1.本科生《微生物工程》《发酵工程实验》《合成生物学》

2.研究生《高级生物生物信息学》《论文写作指导与专业外语》

二. 指导研究生情况：

1.合作指导硕士研究生4人（毕业2人，在读2人，发表SCI 4篇），

2.合作指导博士研究生2人（在读，SCI2篇）；

1.发酵工程：

微生物资源开发与利用；功能微生物机理验证及应用开发；混菌发酵模拟及其机制解析；

2 酶工程：

微生物源胰蛋白酶分子改造，几丁质降解酶系开发及利用；微生物负调控蛋白分子改造；

一. 一作及通讯作者文章：

(1).Xing Wang, Zemin Wang, Shaochen Su, Ying Wu , Jingjing Fan, Xiaoxiao Hou, Kunyue Zhang, El-Sayed Salama, Saurabh Kulshrestha, Zhenmin Ling (*), Pu Liu*, Xiangkai Li. Probiotics Pediococcus acidilactici GR-1 promotes the functional strains and remodels gut microbiota to reduce the Cr(VI) toxicity in a dual-chamber simulated intestinal system. Chemosphere, 2024, 356:141927.

(2).Yanli Chen, Zhenmin Ling(#), Xing Wang, Simin Zong, Jinfeng Yang, Qing Zhang,Jing Zhang, Xiangkai Li(*).The beneficial mechanism of chitosan and chitooligosaccharides in the intestine on different health status.Journal of Functional Foods, 2022,97:105232.

(3)Yanli Chen,Zhenmin Ling(#),Tursunay Mamtimin,Aman Khan,Liang Peng,Jinfeng Yang, Gohar Ali,Tuoyu Zhou,Qing Zhang,Jing Zhang,Xiangkai Li(*).Chitooligosaccharides production from shrimp chaff in chitosanase cell surface display system.Carbohydrate Polymers.2022,277:118894.

(4)Gohar Ali,Zhenmin Ling(#)(*),Irfan Saif,Muhammad Usman,Mohammed Jalalah,Farid A.Harraz,M.S.Al-Assiri,El-Sayed Salama,Xiangkai Li.Biomethanation and microbial community response during agricultural biomass and shrimp chaff digestion.Environmental Pollution.2021,278:116801.

(5)Zhenmin Ling(#),Nandini Thakur,Marwa M.El-Dalatony,El-Sayed Salama(*),XiangkaiLi1(*),Protein biomethanation:insight into the microbial nexus.Trend in Microbiology.2022,30(1):69-78.

(6)HuawenHana,Zhenmin Ling(#),Aman Khan,Amanpreet Kaur Virk,Saurabh Kulshrestha,Xiangkai Li(*).Improvements of thermophilic enzymes:From genetic modifications to applications.BioresourceTechnology.2019.279:350-361.

(7)Ning Yang, Zhenmin Ling(#),Liang Peng,Yanlai Liu,Pu Liu,Kai Zhang,Aman Khan,Juanjuan Shi1,Xiangkai Li(*).Improvement of Enzymatic Stability and Catalytic Efficiency of Recombinant Fusarium oxysporum Trypsin with Different N-Terminal Residues Produced by Pichia pastoris.Journal of Microbiology and Biotechnology.2018,28(9):1482-1492.

(8)Yuefeng Zhang(#),Zhenmin Ling(#),Guocheng Du(*),Jian Chen,Zhen Kang(*).Improved Production of Active Streotomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy. Scientfic Reports.2016,6:2158.  DOI:10.1038/srep23158

(9)Zhenmin Ling(#),Zhen Kang(*),Yi Liu,Song Liu,Jian Chen,Guocheng Du(*).Improvement of Catalytic efficiency and thermotability of recombinant Streptomyces griseus trypsin by introducing artificial propeptide. World Journal of Microbiology and Biotechnology.2014,30(6):1819-1827.

(10)Ling Zhenmin(#),Liu Yi,Teng Shaolei,Kang Zhen(*),Zhang Jingjing,Chen Jian,Du Guocheng(*).Rational design of a novel propeotide for improving active production of Steptomyces griseus trypsin in Pichia pastoris. Applied and Environmental Microbiology.2013,79(12):3851-3855.

(11)Ling Zhenmin(#),Ma Tengbo,Li Jianghua,Du Guocheng,Kang Zhen(*),Chen Jian(*).Functonal expression of trypsin from Streptomyces griseus by Pichia pastoris. Journal of Industrial Microbiology and Biotechnology.2012,39(11):1651-1662.

二. 其他论文

(1)Jinfeng Yang, Pengya Feng, Zhenmin Ling(#), Aman Khan , Xing Wang,Yanli Chen, Gohar Ali, Yitian Fang, El-Sayed Salama, Ximei Wang, Pu Liu(*), Xiangkai Li(*).Nickel exposure induces gut microbiome disorder and serum uric acid elevation. Environmental Pollution.2023,324:121349.

(2)Rong Han,Aman Khan,Zhenmin Ling,Ying Wu,Pengya Feng,Tuoyu Zhou,El-Sayed Salama,Marwa M. El-Dalatony,Xirong Tian,Pu Liu(*),Xiangkai Li.Feed-additive Limosilactobacillus fermentum GR-3 reduces arsenic accumulation in Procambarus clarkii.Ecotoxicology and Environmental Safety.2022,231:113216.

(3)Pengya Feng,Ze Ye,Huawen Han,Zhenmin Ling,Tuoyu Zhou,Shuai Zhao,Amanpreet KaurVirk,Apurva Kakade,AbdEl-Fatah Abomohra,Marwa M.El-Dalatony,EI-Sayed Salama,Pu Liu1(*),Xiangkai Li(*).Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota.Communication Biology.2020,3:242.

(4)Junjiao Zhang,Zhen Kang(*),Zhenmin Ling,Wenlong Cao,Long Liu,Miao Wang,Guocheng Du,Jian Chen(*).High-level extracellular production of alkaline polygalcturonate lyase in Bacillus subtilis with optimized regulatoy elements.Bioresource Technology.2013,146:543-548.

(5)马腾博，令桢民，康振，李江华，堵国成，陈坚(*).灰色链霉菌胰蛋白酶在变铅青链霉菌中的异源表达及酶学性质分析.生物工程学报.2013,9(4):466-479.