主持国家自然科学基金青年基金1项；

主持甘肃省科技厅面上项目1项；

主持教育部中央高校基本科研业务费面上项目3项；

参与国家自然科学基金3项。

指导硕士生9人，5人已毕业，协助指导博士生3名（已毕业，高校任职）；

主讲本科生课程《结构生物学》、《生命伦理学》；

主讲研究生课程《电镜结构生物学》、《结构生物学导论》。

本实验室主要应用分子生物学、生物化学、冷冻电镜三维重构技术等技术手段对以下问题进行研究：

1. 炎症标识蛋白CRP在进化过程中功能差异与结构的关系；

2. 先天免疫中发挥重要作用的补体蛋白与其调控蛋白互作的分子机制，病原体入侵宿主致病的分子机制；

3. 致病菌侵染宿主的结构基础及分子机制。

#-First author, *-Corresponding author.

● Bin Cheng#, Yu-Long Tang, Ya-Fei Gou, Jing-Yi Li, Tian-Hao Xu, Li Zhu*, Efficient expression and purification of rat CRP in Pichia pastoris, Front Immunol., 2024, 15:1465365.

● Shen Li# , Tianhao Xu# , Xinru Meng, Yujuan Yan, Ying Zhou, Lei Duan, Yulong Tang, Li Zhu*, Litao Sun*, Ocr-mediated suppression of BrxX unveils a phage counter-defense mechanism, Nucleic Acids Research, 2024, 52(14):8580-8594.

● Xinxing Zheng#, Chenchen Su, Lei Duan, Mengqi Jin, Yongtao Sun, Li Zhu*, Wenhua Zhang*, Molecular basis of A. thaliana KEOPS complex in biosynthesizing tRNA t6A, Nucleic Acids Research, 2024, 1–18.

● Wenbo He^#, Ke Wu^#, Zhenlin Ouyang, Yixin Bai, Wen Luo, Di Wu, Hao An, Yucheng Guo, Min Jiao, Qian Qin, Jiaxin Zhang, Yi Wu, Junjun She, Peter M. Hwang, Fang Zheng, Li Zhu*, Yurong Wen*, Structure and assembly of type VI secretion system cargo delivery vehicle, Cell Reports, 2023, 42(7):112781.

● Ji S.-R.*, Zhang S.-H., Chang Y., Li H.-Y., Wang M.-Y., Lv J.-M., Zhu L., Tang P.M.K., Wu Y.*, C-reactive protein: the most familiar stranger. Journal of Immunology, 2023, 210(6): 699-707.

● Wang M.-Y., Zhang C.-M., Zhou H.-H., Ge Z.-B., Su C.-C., Lou Z.-H., Zhang X.-Y., Xu T.-T., Li S.-Y., Zhu L., Zhou Y.-L., Wu Y.*, Ji S.-R.*, Identification of a distal enhancer that determines the expression pattern of acute phase marker C-reactive protein. Journal of Biological Chemistry, 2022, 298(8): 102160.

● Yonggang Lu*, Jiaxuan Liu, Li Zhu*, Bianlan Zhang, Jing He, 3D reconstruction from cryo-EM projection images using two spherical embeddings, Communications Biology, 2022, 5:304

● Cheng B., Lv J.-M., Liang Y.-L., Zhu L., Huang X.-P., Li H.-Y., Potempa L.A., Ji S.-R.*, Wu Y.*, Secretory quality control constrains functional selection-associated protein structure innovation. Communications Biology, 2022, 5: 268.

● Bin Cheng#, Di Wu, Ke Wu, Xiao-Ping Huang, Jian-Min Lv, Shang-Rong Ji, Li Zhu*, Purification of Recombinant Mouse C-Reactive Protein from Pichia Pastoris GS115 by Nickel Chelating Sepharose Fast-Flow Affinity Chromatography and P-Aminophenyl Phosphoryl Choline Agarose Resin Affinity Chromatography in Tandem, Journal of Chromatographic Science, 2021, DOI: 10.1093/chromsci/bmab121

● Ke Wu#, Di Wu, Li Zhu*, Yi Wu*, Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation, Int. J. Mol. Sci. 2021, 22(16), 8940.

● Zhenlin Ouyang#, Fang Zheng#, Li Zhu#, Jan Felix, Di Wu, Ke Wu, Irina Gutsche, Yi Wu, Peter M. Hwang, Junjun She, Yurong Wen, Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS, iScience, 2021, 24(5):102476.

● Ying Liu#, Bin Yuan#, Liang Peng, Jing Zhao, Bin Cheng, Yuhua Huang, Xinxing Zheng, Yuerong Zhou, Song Xiang, Li Zhu*, Yi Wu*, Single‐particle analysis of urea amidolyase reveals its molecular mechanism, Protein Science, 2020, 29(5): 1242-1249.

● Kun Wang, Yanjun Shen, Yongzhi Yang, Xiaoni Gan, Guichun Liu, Kuang Hu, Yongxin Li, Zhaoming Gao, Li Zhu, Guoyong Yan, Lisheng He, Xiujuan Shan, Liandong Yang, Suxiang Lu, Honghui Zeng, Xiangyu Pan, Chang Liu, Yuan Yuan, Chenguang Feng, Wenjie Xu, Chenglong Zhu, Wuhan Xiao, Yang Dong, Wen Wang*, Qiang Qiu*, Shunping He*, Morphology and genome of a snailfish from the Mariana Trench provide insights into deep-sea adaptation. Nature Ecology & Evolution, 2019, 3:823–833.

● Zhao J, Zhu L, Fan C, Wu Y, Xiang S*, Structure and function of urea amidolyase. Biosci Rep., 2018 Jan 17;38(1). pii: BSR20171617. doi: 10.1042/BSR20171617.

● Xiong J, Liu X, Gong Y, Zhang P, Qiang S, Zhao Q, Guo R, Qian Y, Wang L, Zhu L, Wang R, Hao Z, Wen H, Zhang J, Tang K, Zang WF, Yuchi Z, Chen H, Wayne Chen SR, Zheng W, Wang SQ, Xu YW, Liu Z., Pathogenic mechanism of a catecholaminergic polymorphic ventricular tachycardia causing-mutation in cardiac calcium release channel RyR2, J Mol Cell Cardiol,2018, 117: 26-35 (2016 IF 5.68).

● Yan Ouyang, Li Zhu*, Yifang Li, Miaomiao Guo, Yang Liu, Jin Cheng, Jing Zhao, Yi Wu*, Architectural plasticity of AMPK revealed by electron microscopy and X-ray crystallography, Scientific Reports, 2016, 6:24191, DOI: 10.1038/srep24191 (co-corresponding author) (2016 IF 4.259)

● Zhu Li#, Zhong Xiaowei, Chen S R Wayne, Banavali Nilesh, Liu Zheng*, Modeling a ryanodine receptor N-terminal domain connecting the central vestibule and the corner clamp region, Journal of Biological Chemistry, 2013, 288(2): 903-914 (5-Year IF 4.863)

● Zhong Xiaowei#, Liu Ying#, Zhu Li#, Meng Xing, Wang Ruiwu, Van Petegem Filip, Wagenknecht Terence, Chen S R Wayne, Liu Zheng*, Conformational dynamics inside amino-terminal disease hotspot of ryanodine receptor, Structure, 2013, 21(11): 2051-2060 (co-first author) (5-Year IF 6.337)

● Zhu Li#, Chen Lei, Zhou Xiao-Ming, Zhang Yuan-Yuan, Zhang Yi-Jiong, Zhao Jing, Ji Shang-Rong, Wu Jia-Wei*, Wu Yi*, Structural insights into the architecture and allostery of full-length AMP-activated protein kinase, Structure, 2011, 19(4): 515-522 (5-Year IF 6.337)

● Ji S.-R., Wu Y.*, Zhu L., Potempa L.A., Sheng F.-L., Lu W., Zhao J. (2007) Cell membranes and liposomes dissociate C-reactive protein (CRP) to form a new, biologically active structural intermediate: mCRPm. FASEB J, 21(1): 284-294. (5-Year IF 6.045)