1. 国家自然科学基金-面上项目，32170330，拟南芥SEC1A调控花粉管极性生长的分子机制研究，2022/01-2025/12，主持

2. 国家自然科学基金-面上项目，31970195，拟南芥P4-ATPase的β亚基成员ALIS1/3调控根发育的功能研究，2020/01-2023/12，主持

3. 国家自然科学基金-优秀青年科学基金项目，31722005，植物细胞骨架，2018/01-2020/12，主持

4. 国家自然科学基金-面上项目，31670180，拟南芥微丝解聚因子第三亚家族成员生理生化功能研究，2017/01-2020/12，主持

5. 国家自然科学基金-面上项目，31470283，拟南芥微丝交联蛋白CROLINs调控花粉管生长的分子机理研究，2015/01-2018/12，主持

6. 教育部"新世纪优秀人才支持计划"，NCET-13-0264，植物细胞骨架，2014/01-2015/12，主持

7. 国家自然科学基金-面上项目，31270326，拟南芥液泡氢ATP酶B亚基不同异形体的生理生化功能及作用机理研究，2013/01-2016/12，主持

8. 甘肃省杰出青年基金，2013GS10064，微丝骨架参与植物响应干旱和高盐胁迫分子机理研究，2013/01-2015/12，主持

9. 国家自然科学基金-青年科学基金，30800079，拟南芥膜联蛋白annexin5调控花粉管极性生长的分子机制研究，2009/01-2011/12，主持

有性生殖是被子植物繁衍后代的关键生理活动，也是农作物种子形成的基础。花粉萌发和花粉管极性生长对于被子植物完成双受精至关重要，该过程涉及到细胞骨架重塑、囊泡定向运输、细胞间通讯、信号转导等重要的细胞活动，其也是研究植物细胞极性建立和生长的理想模式材料之一。此外，随着全球气候变暖，玉米、棉花等农作物在散粉期频频出现极端高温天气，导致农作物花粉活力下降和丧失，从而造成种子败育甚至绝收，已经严重影响了我国重要农作物的产量。目前，有关花粉管极性生长与响应环境的调控机制一直是植物学研究领域的热点和重点。本课题组主要以拟南芥和玉米为实验材料，主要开展如下研究工作：

1. 植物细胞骨架特有的调控机制；

2. 植物细胞极性建立和维持的分子机理；

3. 植物有性生殖过程中的信号转导与调控机制；

4. 植物感知和响应高温胁迫的信号网络；

5. 极端高温影响玉米有性生殖的分子机理；

6. 耐高温玉米种质改良和新品种培育。

1. 甘肃省领军人才（2020）

2. 国家优秀青年基金获得者（2017）

3. 教育部“长江学者奖励计划”青年学者（2017）

4. 教育部"新世纪优秀人才支持计划"（2013）

5. 甘肃省杰出青年基金（2013）

*代表通讯作者，#代表共同第一作者

[36] Dong Qian^#, Tian Li^#, Chen Zheng^#, Yue Niu, Yingzhi Niu, Chengying Li, Muxuan Wang, Yang Yang, Lizhe An, Yun Xiang*. (2024). Actin-Depolymerizing Factors 8 and 11 Promote Root Hair Elongation at High pH. Plant Communications, 5(3): 100787.

[35] Dong Qian^#, Tian Li^#, Shuyuan Chen^#, Dongshi Wan, Yongxing He, Chen Zheng, Jiajing Li, Zhenping Sun, Jiejie Li, Junxia Sun, Yingzhi Niu, Hongxia Li, Muxuan Wang, Yue Niu, Yang Yang, Lizhe An, Yun Xiang*. (2024). Evolution of the thermostability of actin-depolymerizing factors enhances the adaptation of pollen germination to high temperature. The Plant Cell, 36(4): 881-898.

[34] Hongxia Li^#, Yang Yang^#, Hongkai Zhang, Chengying Li, Pingzhou Du, Mengmeng Bi, Tao Chen, Dong Qian, Yue Niu, Haiyun Ren, Lizhe An, Yun Xiang*. (2023). The Arabidopsis GPI-anchored protein COBL11 is necessary for regulating pollen tube integrity. Cell Reports 42, 113353.

[33] Tingting Fan^#, Yuemin Fan^#, Yang Yang, Dong Qian, Yue Niu, Lizhe An, Yun Xiang*. (2023). SEC1A and SEC6 synergistically regulate pollen tube polar growth. Journal of Integrative Plant Biology 65(7):1717-1733.

[32] Pingzhou Du, Yu Liu, Lu Deng, Dong Qian, Xiuhua Xue, Ting Yang, Tonghui Li, Yun Xiang*, Haiyun Ren*. (2023). AtMAC stabilizes the phragmoplast by crosslinking microtubules and actin filaments during cytokinesis. Journal of Integrative Plant Biology 65(8):1950-1965.

[31] Yumei Shi, Changxin Luo, Yun Xiang, Dong Qian* (2023). Rab GTPases, tethers, and SNAREs work together to regulate Arabidopsis cell plate formation. Frontiers in Plant Science, 14: 1120841.

[30] Yang Yang^#, Yue Niu^#, Tao Chen, Hongkai Zhang, Jingxia Zhang, Dong Qian, Mengmeng Bi, Yuemin Fan, Lizhe An, Yun Xiang*. (2022). The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis. The Plant Cell 34, 3718-3736. (Highlighted paper by Science Editor in “In Brief”)

· Vera Gorelova, ALA3 regulates pollen tube growth and guidance by keeping PRKs in all the right places, The Plant Cell, 2022, 34(10): 3497–3498.

[29] Zhongcai Jin, Tian Li, Yuelong Zhou, Yuanzhi Huang, Chengqing Ning, Jing Xu, Glenn Hicks, Natasha Raikhel, Yun Xiang, Ruixi Li* (2022). Small molecule RHP1 promotes root hair tip growth by acting upstream of the RHD6-RSL4-dependent transcriptional pathway and ROP signaling in plants. The Plant Journal 110:1636-1650.

[28] Changxin Luo, Yumei Shi, Yun Xiang*. (2022). SNAREs regulate vesicle trafficking during root growth and development. Frontiers in Plant Science, 14: 13:853251.

[27] Zhiqi Liu, Jiayang Gao, Yong Cui, Sven Klumpe, Yun Xiang, Philipp S Erdmann, Liwen Jiang*. (2021). Membrane imaging in the plant endomembrane system. Plant Physiology, 185:562-576.

[26] Pan Zhang, Dong Qian, Changxin Luo, Yingzhi Niu, Tian Li, Chengying Li, Yun Xiang, Xinyu Wang, Yue Niu*. (2021). Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress. Frontiers in Cell and Developmental Biology, 9:635533.

[25] Yuelong Zhou^#, Yang Yang^#, Yue Niu^#, Tingting Fan, Dong Qian, Changxin Luo, Yumei Shi, Shanwei Li, Lizhe An, Yun Xiang*. (2020). The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth. The Plant Cell, 32: 3170-3187.

[24] Dong Qian^* and Yun Xiang. (2019). Actin cytoskeleton as actor in upstream and downstream of calcium signaling in plant cells. International Journal of Molecular Sciences, 20: 1403.

[23]钱东，李盼盼，向云*.微丝骨架调控植物特有生理活动的研究进展.中国细胞生物学学报, 2019, 41: 399–405. (创刊四十周年专栏·细胞骨架)

[22] Dong Qian, Zhe Zhang, Juanxia He, Pan Zhang, Xiaobin Ou, Tian Li, Lipan Niu, Qiong Nan, Yue Niu, Wenliang He, Lizhe An, Kun Jiang, and Yun Xiang^*. (2019). Arabidopsis ADF5 promotes stomatal closure by regulating actin cytoskeleton remodeling in response to ABA and drought stress. Journal of Experimental Botany, 70: 435–446.

[21] Zhonghao Jiang, Xiaoping Zhou, Ming Tao, Fang Yuan, Lulu Liu, Feihua Wu, Xiaomei Wu , Yun Xiang, Yue Niu, Feng Liu, Chijun Li , Rui Ye, Benjamin Byeon, Yan Xue, Hongyan Zhao, Hsin-Neng Wang, Bridget Crawford, Douglas Johnson, Chanxing Hu, Christopher Pei, Wenming Zhou, Gary Swift, Han Zhang, Tuan Vo-Dinh, Zhangli Hu*, James Siedow*, Zhen-Ming Pei* (2019) Plant cell-surface GIPC sphingolipids sense salt to trigger Ca²⁺ influx. Nature 572: 341-346.

[20] Yue Niu* and Yun Xiang*. (2018). An overview of biomembrane functions in plant responses to high-temperature stress. Frontiers in Plant Science, 9: 915.

[19] 钱东，向云*. 植物特有微丝结合蛋白研究进展.中国科学：生命科学. 2017, 47: 829-838.（兰州大学专刊）

[18] Yue Niu*, Dong Qian, Baiyun Liu, Jianchao Ma, Dongshi Wan, Xinyu Wang, Wenliang He, Yun Xiang*. (2017). ALA6, a P4-type ATPase, Is Involved in Heat Stress Responses in Arabidopsis thaliana. Frontiers in Plant Science, 8: 1732.

[17] Hui Su*, Hualing Feng, Xiaoting Chao, Xia Ding, Qiong Nan, Chenxi Wen, Huidong Liu, Sinuo Liu, Yun Xiang*, Wenzhe Liu*. (2017). Fimbrins 4 and 5 Act Synergistically During Polarized Pollen-tube Growth to Ensure Fertility in Arabidopsis. Plant and Cell Physiology, 58: 2006-2016.

[16] Jingen Zhu^#, Qiong Nan^#, Tao Qin^#, Dong Qian, Tonglin Mao, Shunjie Yuan, Xiaorong Wu, Yue Niu, Qifeng Bai, Lizhe An, Yun Xiang*. (2017). Higher-ordered Actin Structures Remodeled by Arabidopsis ACTIN-DEPOLYMERIZING FACTOR5 Are Important for Pollen Germination and Pollen Tube Growth. Molecular Plant, 10: 1065-1081.

[15] Qiong Nan^#, Dong Qian^#, Yue Niu, Yongxing He, Shaofei Tong, Zhimin Niu, Jianchao Ma, Yang Yang, Lizhe An, Dongshi Wan*, Yun Xiang*. (2017). Plant Actin-depolymerizing Factors Possess Opposing Biochemical Properties Arising from Key Amino Acid Changes Throughout Evolution. The Plant Cell, 29(2): 395-408. (Highlighted paper by Science Editor in “In Brief”)

· Jennifer Lockhart, Loose-Knit Family: Tracing the Evolution of Actin-Depolymerizing Factors That Sever or Join the Actin Cytoskeleton. The Plant Cell, 2017, 29 (2): 200-201.

[14] Jiangtao Liu, Yuelong Zhou, Changxin Luo, Yun Xiang, Lizhe An*. (2016). De Novo Transcriptome Sequencing of Desert Herbaceous Achnatherum splendens (Achnatherum) Seedlings and Identification of Salt Tolerance Genes. Genes, 23: 7(4): 12.

[13] Hui Wang, Shengcheng Han, Wei Siao, Chunqing Song, Yun Xiang, Xiaorong Wu, Pengyu Cheng, Hongjuan Li, Ján Jásik, Karol Mičieta, Ján Turňa, Boris Voigt, František Baluška, Jin Liu, Yingdian Wang, Heping Zhao*. (2015). Arabidopsis Synaptotagmin 2 Participates in Pollen Germination and Tube Growth and Is Delivered to Plasma Membrane via Conventional Secretion. Molecular Plant, 8(12):1737-1750.

[12] Dong Qian, Qiong Nan, Yang Yang, Hui Li, Yuelong Zhou, Jingen Zhu, Qifeng Bai, Pan Zhang, Lizhe An, Yun Xiang*. (2015). Gelsolin-Like Domain 3 Plays Vital Roles in Regulating the Activities of the Lily Villin/Gelsolin/Fragmin Superfamily. PLoS One, 10(11): e0143174.

[11] Jingen Zhu, Xiaorong Wu, Shunjie Yuan, Dong Qian, Qiong Nan, Lizhe An, Yun Xiang*. (2014). Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca²⁺-Dependent Membrane Trafficking. PLoS One, 9(7): e102407.

[10] Jingen Zhu, Shunjie Yuan, Guo Wei, Dong Qian, Xiaorong Wu, Honglei Jia, Mengyuan Gui, Wenzhe Liu, Lizhe An*, Yun Xiang*. (2014). Annexin5 is Essential for Pollen Development in Arabidopsis. Molecular Plant, 7(4): 751-754.

[9] Honglei Jia, Jisheng Li, Jingen Zhu, Tingting Fan, Dong Qian, Yuelong Zhou, Jue Wang, Haiyun Ren, Yun Xiang*, Lizhe An*. (2013). Arabidopsis CROLIN1, a Novel Plant Actin-binding Protein, Functions in Cross-linking and Stabilizing Actin Filaments. Journal of Biological Chemisty, 288: 32277-32288.

[8] Tingting Fan, Huanhuan Zhai, Wenwei Shi, Jue Wang, Honglei Jia, Yun Xiang*, Lizhe An*. (2013). Overexpression of Profilin 3 Affects Cell Elongation and F-actin Organization in Arabidopsis thaliana. Plant Cell Reports, 32(1): 149-160.

[7] Binyun Ma, Dong Qian, Qiong Nan, Chang Tan, Lizhe An*, Yun Xiang*. (2012). Arabidopsis V-ATPase B Subunits Are Involved in Actin Cytoskeleton Remodeling via Binding to, Bundling and Stabilizing F-actin. Journal of Biological Chemisty, 287: 19008-19017.

[6] Jue Wang, Dong Qian, Tingting Fan, Honglei Jia, Lizhe An*, Yun Xiang*. (2012). Arabidopsis Actin Capping Protein (AtCP) Subunits Have Different Expression Patterns, and Downregulation of AtCPB Confers Increased Thermotolerance of Arabidopsis after Heat Shock Stress. Plant Science, 193: 110-119.

[5] Shanjin Huang, Yun Xiang, Haiyun Ren. (2011). Actin-binding Proteins and Actin Dynamics in Plant Cells. In The Plant Cytoskeleton, pp. 57-80. Springer New York.

[4] Binyun Ma, Yun Xiang, Lizhe An*. (2011). Structural Bases of Physiological Functions and Roles of the Vacuolar H⁺-ATPase. Cellular Signalling, 23: 1244-1256.

[3] Ting Wang^#, Yun Xiang^#, Jian Hou, Haiyun Ren*. (2008). ABP41 is Involved in the Pollen Tube Development via Fragmenting Actin Filaments. Molecular Plant, 1(6): 1048-1055.

[2] Haiyun Ren*, Yun Xiang. (2007). The Function of Actin-binding Proteins in Pollen Tube Growth. Protoplasma, 230(3-4): 171-182.

[1] Yun Xiang, Xi Huang, Ting Wang, Yan Zhang, Qinwen Liu, Patrick J Hussey, Haiyun Ren*. (2007). ACTIN BINDING PROTEIN29 from Lilium Pollen Plays an Important Role in Dynamic Actin Remodeling. The Plant Cell, 19(6): 1930-1946.