研究团队已引进2名高级研究人员(陈玉辉，副教授；张泽勇，副教授）和1名博士后（马燕林，博士）。目前已招收4名博士研究生（何娟霞，李鹏，沈怡彤, 张锐）和8名硕士研究生（严泽章，邓芬芬，李娟娟，孙承慧，宋小潘，伊苏，孙娟，杨世倩）。

本研究团队每年拟招收一定名额的分子生物学和植物学方向的博士及硕士研究生，师资博士后，以及优秀青年研究员，欢迎广大优秀学子和研究人员联系，报考和加盟本团队。

本研究团队注重豆科植物生长发育调控和共生固氮机理方面的研究。研究方向包括下面几个领域。

1. 叶型调控机理。作为光合作用的主要器官，叶片的生长，形状和类型等指标对植物产量起重要的调控作用。大部分豆科植物的叶型属于复叶。几年前，我们对调控豆科植物复叶发育的分子机理的了解还是非常有限。利用遗传和分子生物学手段，我们从豆科模式植物，蒺藜苜蓿（Medicago truncatula）中分离和分析了多个重要的调控基因（SGL1，PALM1，FCL1，ELP1， PHAN, PIN10）。现期工作利用遗传，分子生物学，生物化学等手段，分离调控复叶叶型发育的新基因，分析它们的作用机理， 以及阐明它们与已知基因、信号传导途径的关系。

2. 植物器官生长调控机理。 植物营养和生殖器官的生长受植物本身的发育途径和外界环境的影响。在发育过程中，植物器官的生长受细胞分裂和分化的活性和周期调控。利用遗传和转录表达分析，我们从蒺藜苜蓿中分离到一个调控营养和生殖器官大小的关键蛋白（BS1）。我们的研究表明BS1通过抑制细胞分裂来调控器官生长周期。我们的研究进一步表明在豆科作物大豆中，BS1同源基因与BS1类似，调控营养和生殖器官的大小。现期工作利用分子生物学进一步研究BS1调控细胞分裂的机理。另外，利用分子生物学和遗传学的手段，解析BS1和已知的调控器官生长的蛋白的相互作用。

3. 共生固氮。豆科植物能与土壤固氮菌通过一系列信号传导过程，建立共生关系，形成全新的器官(根瘤)。在根瘤侵染细胞中，固氮菌将空气中的氮气转化成宿主可利用的氨。这个过程需要消耗大量宿主提供的能量和碳源。因此，宿主对根瘤的数目，生物固氮的活性和周期有严格的控制。目前，对豆科植物-根瘤菌前期信号传导途径已经有比较深入的了解。但是，对后期的信号传导途径，宿主细胞和根瘤菌的共生关系方面的了解还是非常有限。本研究团队在过去十几年里通过对蒺藜苜蓿突变体文库的筛选，已经分离出大量的共生固氮方面的突变体。现期工作利用遗传，生化和分子生物学的方法，分离和分析共生固氮方面新的调控基因。另外，对已经分离出的基因进行功能，机理研究。

Publication (*Corresponding author)

Ma, Y., Chen, R.* (2021) N and P signaling and transport during legume-rhizobium symbiosis. Frontiers in Plant Science (Provisionally accepted).

Wang, T., Ren, L., Li, C., Zhang, D., Zhang X., Zhou, G., Gao, D., Chen, R., Chen, Y., Wang, Z., Shi, F., Farmer, A., Li, Y., Zhou, M., Young, N., Zhang, W. (2021) The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress. BMC Biology, 19, 96.

Ng, J., Welvaert, A., Wen, J., Chen, R., Mathesius, U. (2020) The Medicago truncatula PIN2 auxin transporter mediates basipetal auxin transport but is not necessary for nodulation. Journal of Experimental Botany, 71(4), 1562-1573.

Ge, L.* and Chen, R.* (2019) NEGATIVE GRAVITROPIC RESPONSE OF ROOTS directs auxin flow to control root gravitropism. Plant Cell & Environment 42, 2372-2383.

Liu, J., Rutten, L., Limpens, E., van der Molen, T., van Velzen, R., Chen, R., Chen, Y., Geurts, R., Kohlen, W., Kulikova, O., Bisseling T. (2019) A remote cis-regulatory region is required for NIN expression in the pericycle to initiate nodule primordium formation in Medicago truncatula. Plant Cell, 31(1), 68-83.

Chen, R.* (2019) Compound leaf development in Medicago truncatula. In: The Model  Legume Medicago truncatula. Ed. Frans J. DeBruijn (John Wiley & Sons, Inc.), DOI: 10.1002/9781119409144.ch19.

Chen, Y., Xi, J. and Chen, R.* (2019) Medicago truncatula ESN1, a key regulator of nodule development and symbiotic nitrogen fixation. In: The Model Legume Medicago truncatula. Ed. Frans J. DeBruijn (John Wiley & Sons, Inc.), DOI: 10.1002/9781119409144.ch87.

Kontra-Kováts, G., Fodor, L., Horváth, B., Domonkos, Á., Iski, G., Chen, Y., Chen, R. and Kaló, P. (2019) Isolation and characterization of the non-transposon symbiotic nitrogen fixing mutants of Medicago truncatula. In: The Model Legume Medicago truncatula. Ed. Frans J. DeBruijn (John Wiley & Sons, Inc.), DOI: 10.1002/9781119409144.ch129.

Chen, R.* (2018) Functional genomics and genetic control of compound leaf development in Medicago truncatula: an overview. Methods in Molecular Biology, 1822, 197-203.

Chen, Y. and Chen, R.* (2018) Physical mutagenesis in Medicago truncatula using Fast Neutron Bombardment (FNB) for symbiosis and developmental biology studies. Methods in Molecular Biology, 1822, 61-69.

Xi, J., Chen, H., Bai, W., Yang, R., Yang, P., Chen, R., Hu, T. and Wang, S. (2018) Sodium-related adaptation to drought: New insights from the xerophyte plant Zygophyllum xanthoxylum. Frontiers in Plant Science, doi: 10.3389/fpls.2018.01678.

Chen, Y., Wang, X., Lu, S., Li, S. and Chen, R.* (2017) An array-based comparative genomic hybridization platform for detection of copy number variations in fast neutron induced Medicago truncatula mutants. Journal of Visualized Experiments, http://www.jove.com/video/56470.

Wang, T., Zhao, M., Zhang, X., Liu, M., Yang, C., Chen, Y., Chen, R., Wen, J., Mysore, K., Zhang, W. (2017) Novel phosphate deficiency-responsive long non-coding RNAs in a legume model plant Medicago truncatula. Journal of Experimental Botany, 68(21-22): 5937-5948.

Peng, J., Berbel, A., Madueno, F. and Chen, R.* (2017) AUXIN RESPONSE FACTOR3 regulates compound leaf patterning by directly repressing PALMATE-LIKE PENTAFOLIATA1 expression in Medicago truncatula. Frontiers in Plant Science, doi; 10.3389/fpls.2017.01630.

Liao, F., Peng, J. and Chen, R.* (2017) LeafletAnalyzer, an automated software for quantifying, comparing and classifying blade and serration features of compound leaves during development, and among induced mutants and natural variants in the legume Medicago truncatula. Front. Frontiers in Plant Science, 10.3389/fpls.2017.00915.

Ge, L., Yu, J., Wang, H., Luth, D., Bai, G., Wang, K. and Chen, R.* (2016) Increasing seed size and quality by manipulating BIG SEEDS1 gene in legumes. Proc. Natl. Acad. Sci. U. S. A., 113: 12414-12419.

Ge, L. and Chen, R.* (2016) Negative gravitropism in plant roots. Nature Plants, 2: 16155.

Wang, T.-Z., Liu, M., Zhao, M-G., Chen, R. and W.-H. Zhang (2015) Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing. BMC Plant Biology, 15(1): 131.

Paez-Garcia, A., Motes, C. M., Scheible, W-R., Chen, R., Blancaflor, E. B. and M.J. Monteros (2015) Root traits and phenotyping strategies for plant improvement. Plants, 4(2): 334-355.

Kim, M., Chen, Y., Xi, J., Waters, C., Chen, R. and Wang, D. (2015) An antimicrobial peptide essential for bacterial survival in the nitrogen-fixing symbiosis. Proc. Natl. Acad. Sci. U. S. A., 112(49): 15238-15243.

Horváth, P., Domonkos, Á., Kereszt, A., Szűcs, A., Ábrahám, E., Ayaydin, F., Bóka, K., Chen, Y., Chen, R., Murray, J., Udvardi, M., Kondorosi, É., Kaló, L. (2015) Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant. Proc. Natl. Acad. Sci. U. S. A., 112(49): 15232-15237.

Lauressergues, D., André, O., Peng, J., Wen, J., Chen, R., Ratet, P., Tadege, M., Mysore, K. S and S. F. Rochange (2015) Strigolactones contribute to shoot elongation and to the formation of leaf margin serrations in Medicago truncatula R108. Journal of Experimental Botany, 66(5): 1237-1244.

Jha, A.K., Wang, Y., Hercyk, B. S., Shin, H-S, Chen, R. and M. Yang (2014) The role for CYCLIN A1; 2/TARDY ASYNCHRONOUS MEIOSIS in differentiated cells in Arabidopsis. Plant Molecular Biology, 85(1-2): 81-94.

Ge, L., Peng, J., Berbel, A., Madueño, F. and Chen, R.* (2014) Regulation of compound leaf development by PHANTASTICA in Medicago truncatula. Plant Physiology, 164(1): 216-228.

Ge, L. and Chen, R.* (2014) PHANTASTICA regulates leaf polarity and petiole identity in Medicago truncatula. Plant Signaling & Behavior, 9(3): e28121.

Xi, J., Chen, Y., Nakashima, J., Wang, S-M. and Chen, R.* (2013) Medicago truncatula esn1 defines a genetic locus involved in nodule senescence and symbiotic nitrogen fixation. Molecular Plant-Microbe Interactions, 26(8): 893-902.

Wang, Y. and Chen, R.* (2013) Regulation of Compound Leaf Development. Plants, 3(1): 1-17.

Peng, J., Ge, L., Wang, Y. and Chen, R.* (2013) Signaling and transport of auxin and plant development. In: Polar Auxin Transport, eds. Chen, R. and Baluska F., Springer-Verlag Berlin Heidelberg, 239-258.

Uppalapati, S.R., Ishiga, Y., Doraiswamy, V., Bedair, M., Mittal, S., Chen, J., Nakashima, J., Tang, Y., Tadege, M., Ratet, P., Chen, R., Schultheiss, H., and K. Mysore (2012) Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens. Plant Cell 24(1): 353-370.

Pislariu, C., Murray, J., Wen, J., Cosson, V., Duvvuru Muni, R., Wang, M., Benedito, V., Andriankaja, A., Cheng, X., Mondy, S., Zhang, S., Taylor, M.; Tadege, M., Ratet, P., Mysore, K., Chen, R. and M. Udvardi (2012) A Medicago truncatula tobacco retrotransposon insertion mutant collection with defects in nodule development and symbiotic nitrogen fixation. Plant Physiology, 159(4): 1686-1699.

Cheng, X., Peng, J., Ma, J., Tang, Y., Chen, R., Mysore, K.S. and Wen, J. (2012) NO APICAL MERISTEM (MtNAM) regulates floral organ identity and lateral organ separation in Medicago truncatula. New Phytologist, 195(1): 71-84.

Chen, J., Moreau, C., Liu, Y., Kawaguchi, M., Hofer, J., Ellis, N. and Chen, R.* (2012) Conserved genetic determinant of motor organ identity in Medicago truncatula and related legumes. Proc. Natl. Acad. Sci. U. S. A., 109(29): 11723-11728.

Zhou, Z.Y., Zhang, C.G., Wu, L., Chai, J., Wang, M., Jha, A., Jia, P.F., Cui, S.J., Yang, M., Chen, R.* and G.Q. Guo* (2011) Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root. Plant Journal, 66(3): 516-527.

Peng, J., Yu, J., Wang, H., Guo, Y., Li, G., Bai, G. and Chen, R.* (2011) Regulation of compound leaf development in Medicago truncatula by fused compound leaf1, a class M KNOX gene. Plant Cell, 23(11): 3929-3943.

Peng, J. and Chen, R.* (2011) Auxin efflux transporter MtPIN10 regulates compound leaf and flower development in Medicago truncatula. Plant Signaling & Behavior, 6(10): 1537-1544.

Murray, J.D., Duvvuru Muni, R., Torres-Jerez, I., Tang, Y., Allen, S., Andriankaja, M., Li, G., Laxmi, A., Cheng, X., Vaughan, D., Schultze, M., Sun, J., Charpentier, M., Oldroyd, G., Tadege, M., Ratet, R., Mysore, K. S., Chen, R. and Udvardi, M. (2011) Vapyrin, a gene essential for intracellular progression of arbuscular mycorrhizal symbiosis, is also essential for infection by rhizobia in the nodule symbiosis of Medicago truncatula. Plant Journal, 65(2): 244-252.

Wang, Y., Jha, A. K., Chen, R., Doonan, J. H. and M. Yang (2010) Polyploidy‐associated genomic instability in Arabidopsis thaliana. Genesis, 48(4): 254-263.

Chen, J., Yu, J., Ge, L., Wang, H., Berbel, A., Yu, L., Chen, Y., Li, G., Tadege, M., Wen, J., Cosson, V., Mysore, K. S., Ratet, P., Madueno, F., Bai, G. and Chen, R.* (2010) Control of dissected leaf morphology by a Cys (2) His (2) zinc finger transcription factor in the model legume Medicago truncatula. Proc. Natl. Acad. Sci. U. S. A., 107(23): 10754-10759.

Ge, L., Chen, J. and Chen, R.* (2010) Palmate-like pentafoliata1 encodes a novel Cys (2) His (2) zinc finger transcription factor essential for compound leaf morphogenesis in Medicago truncatula. Plant Signaling & Behavior, 5(9): 1134-1137.

Rogers, C., Wen, J., Chen, R. and Oldroyd, G. (2009) Deletion-based reverse genetics in Medicago truncatula. Plant Physiology, 151(3): 1077-1086.

Pan, J., Fujioka, S., Peng, J., Chen, J., Li, G. and Chen, R.* (2009) The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana. Plant Cell, 21(2): 568-580.

Zhou, X., Wang, Y.Z., Smith, J.S. and Chen, R. (2008) Altered expression patterns of TCP and MYB genes relating to the floral developmental transition from initial zygomorphy to actinomorphy in Bournea (Gesneriaceae). New Phytologist, 178: 532-543.

Wang, H., Chen, J., Wen, J., Tadege, M., Li, G., Liu, Yu, Mysore, K. S., Ratet, P. and Chen, R.* (2008) Control of compound leaf development by FLORICAULA/LEAFY ortholog SINGLE LEAFLET1 in Medicago truncatula. Plant Physiology, 146(4): 1759-1772.

Laxmi, A., Pan, J., Morsy, M. and Chen, R.* (2008) Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana. PLoS ONE, 3(1): e1510.

Udvardi, M., Benedito, V., Zhang, J., Murray, J., Torrez-Jerez, I., Chen, R (2007) Genomics of Medicago truncatula: advances and potential use in other species. LOTUS NEWSLETTER, p. 93.

Sedbrook, J., Boonsirichai, K., Chen, R., Hilson, P., Pearlman, R., Rosen, E., Rutherford, R., Battiza, A., Carroll, K., Schulz, T. (2007) Molecular genetics of root gravitropism and waving in Arabidopsis thaliana. Gravitational and Space Research, 2007. 11(2).

Wang, H., Li, G. and Chen, R.* (2006) Fast neutron bombardment (FNB) mutagenesis for forward and reverse genetic studies in plants. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues (1st Ed). J.A.T.d. Silva, ed., 629-639.

Shin, H., Shin, H‐S., Chen, R. and M.J. Harrison (2006) Loss of At4 function impacts phosphate distribution between the roots and the shoots during phosphate starvation. Plant Journal, 45(5): 712-726.

Chen, R.* and P. Masson (2006) Auxin transport and recycling of PIN proteins in plants. In: Plant Endocytosis. p. 139-157.

Zimmermann, P., Hennig, L., Gruissem, W., Baluška, F., Barlow, P. W., Baskin, T. I., Chen, R., Feldman, L., Forde, B. G., Geisler, M., Judy J., Diedrik M., Dieter V., Pierre D., Melinda L-R. and S. O. Prasher (2005) 413 Green plants as intelligent organisms. Trends in Plant Science, 10(9): 407-458.

Shin, H., Shin, H‐S., Guo, Z., Blancaflor, E. B., Masson, P. H. and Chen, R.* (2005) Complex regulation of Arabidopsis AGR1/PIN2‐mediated root gravitropic response and basipetal auxin transport by cantharidin‐sensitive protein phosphatases. Plant Journal, 42(2): 188-200.

Baluška, F., Barlow, P. W., Baskin, T. I., Chen, R., Feldman, L., Forde, B. G., Geisler, M., Jernstedt, J., Menzel, D., Muday, G. K., Murphy, A., Šamaj, J., Taiz, L. and D. Volkmann (2005) What is apical and what is basal in plant root development? Trends in Plant Science, 10(9): 409-411.

Peer, W.A., Bandyopadhyay, A., Blakeslee, J. J., Makam, S. N., Chen, R., Masson, P. H. and A.S. Murphy (2004) Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana. Plant Cell, 16(7): 1898-1911.

Hou, G., Kramer, Victoria L., Wang, Y‐S., Chen, R., Perbal, G., Gilroy, S. and E.B. Blancaflor (2004) The promotion of gravitropism in Arabidopsis roots upon actin disruption is coupled with the extended alkalinization of the columella cytoplasm and a persistent lateral auxin gradient. Plant Journal, 39(1):  113-125.

Boonsirichai, K., Sedbrook, J. C., Chen, R., Gilroy, S. and P.H. Masson (2003) ALTERED RESPONSE TO GRAVITY is a peripheral membrane protein that modulates gravity-induced cytoplasmic alkalinization and lateral auxin transport in plant statocytes. Plant Cell, 15(11): 2612-2625.

Masson, P.H., Tasaka, M., Morita, M. T., Guan, C., Chen, R. and K. Boonsirichai (2002) Arabidopsis thaliana: a model for the study of root and shoot gravitropism. The Arabidopsis Book, 2002: e0043.

Chen, R., Guan, C., Boonsirichai, K. and P.H. Masson (2002) Complex physiological and molecular processes underlying root gravitropism. Plant Molecular Biology, 49(3-4): 305-317.

Boonsirichai, K., Guan, C., Chen, R. and P. Masson (2002) Root gravitropism: an experimental tool to investigate basic cellular and molecular processes underlying mechanosensing and signal transmission in plants. Annual Review of Plant Biology, 53(1): 421-447.

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Book editor

Chen, R. and Baluska, F. (2013) Polar Auxin Transport. Series: Signaling and Communication in Plants. Vol. 17, 2013, VII, 330 p, Berlin, Heidelberg, New York: Springer-Verlag Berlin Heidelberg.

邮箱地址：

rjchen@lzu.edu.cn